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KMID : 0380620100420040414
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Korean Journal of Food Science and Technology
2010 Volume.42 No. 4 p.414 ~ p.419
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The Rapid Detection of Antioxidants from Safflower Seeds (Carthamus tinctorius L.) Using Hyphenated-HPLC Techniques
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Kim Su-Jin
Kim Sang-Min
Kang Suk-Woo
Um Byung-Hun
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Abstract
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Hyphenated-HPLC techniques combine the separation power of HPLC with the structural and bioactivity information provided by NMR, ESI/MS, and an on-line antioxidant screening system. The major advantages over the traditional off-line techniques are rapidity and efficiency. In this study, we used hyphenated HPLC techniques including online HPLC-ABTS, LC-NMR, and LC-MS todirectly identify the major antioxidants of safflower (Carthamus tinctorius L.) seeds. The results demonstrated that the major antioxidant compounds from on-line HPLC-ABTS analysis were identified as 8’-hydroxyarcgenin-4’-O-β-D-glucoside, N-(p-coumaroyl) serotonin, and N-feruloylserotonin. Among them, N-feruloylserotonin accounted for almost 50% of the ABTS radical scavenging activity of the total extract. The results demonstrate that HPLC hyphenated techniques can be used to rapidly screen and structurally identify antioxidants from crude plant extracts.
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KEYWORD
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antioxidants, Carthamus tinctorius L., LC-MS, LC-NMR, on-line HPLC-ABTS system
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